Investigador Principal Universidad de Chile.
Líneas de Investigación:
– Modificaciones del RNA y su rol en la replicación viral y la respuesta pro-inflamatoria (RSR-FVE-MA-DTA)
– La helicasa de RNA DDX3X como factor proviral y conductor de la respuesta pro-inflamatoria (RSR-FVE)
– Separación de fases líquido-liquido durante la replicación viral (FVE-RSR)
– Homeostasis del RNA durante la replicación viral (FVE-RSR)
– Regulación post-transcripcional de la respuesta pro-inflmatoria inducida por VIH-1 en el sistema nervioso central (DTA-RSR)
– Expresión génica y replicación del virus respiratorio sincicial (MA-LT-FVE-RSR)
– Rol del sensor IRE1 durante la replicación de ZIKV (RSR-FVE)
– Virus respiratorios como agentes infecciosos en población pediátrica: factores virales y del hospedero de gravedad (LT-MA)
– Relación entre la microbiota intestinal y el estado inflamatorio en personas que viven con VIH bajo terapia antiretroviral (CC-FVE-RSR)
Publicaciones (artículos originales en revistas WoS, últimos 10 años)
1. Figueroa F, Vega-Gibson A, Catrileo J, Gaete-Argel A, Riquelme-Barrios S, Alonso-Palomares LA, Tapia LI, Valiente-Echeverría F, Soto-Rifo R, Acevedo ML. N6 -Methyladenosine Negatively Regulates Human Respiratory Syncytial Virus Replication.
Front Cell Dev Biol. 2021 Oct 4;9:739445. doi: 10.3389/fcell.2021.739445
2. Rojas M, Luz-Crawford P, Soto-Rifo R, Reyes-Cerpa S, Toro-Ascuy D. The Landscape of IFN/ISG Signaling in HIV-1-Infected Macrophages and Its Possible Role in the HIV-1 Latency.
Cells. 2021 Sep 9;10(9):2378. doi: 10.3390/cells10092378.
3. Bueno SM, Abarca K, González PA, Gálvez NMS, Soto JA, Duarte LF, Schultz BM, Pacheco GA, González LA, Vázquez Y, Ríos M, Melo-González F, Rivera-Pérez D, Iturriaga C, Urzúa M, Domínguez A, Andrade CA, Berrios RV, Canedo-Marroquín G, Covián C, Moreno-Tapia D, Saavedra F, Vallejos OP, Donato P, Espinoza P, Fuentes D, González M, Guzmán P, Muñoz-Venturelli P, Pérez CM, Potin M, Rojas Á, Fasce R, Fernández J, Mora J, Ramírez E, Gaete-Argel A, Oyarzún-Arrau A, Valiente-Echeverría F, Soto-Rifo R, Weiskopf D, Sette A, Zeng G, Meng W, González-Aramundiz JV, Kalergis AM; CoronaVac03CL Study Group. Safety and Immunogenicity of an Inactivated SARS-CoV-2 Vaccine in a Subgroup of Healthy Adults in Chile.
Clin Infect Dis. 2021 Sep 19:ciab823. doi: 10.1093/cid/ciab823
4. Hernández-Díaz T, Valiente-Echeverría F, Soto-Rifo R. RNA Helicase DDX3: A Double-Edged Sword for Viral Replication and Immune Signaling.
Microorganisms. 2021 Jun 3;9(6):1206. doi: 10.3390/microorganisms9061206
5. Peña M, Ampuero M, Garcés C, Gaggero A, García P, Velasquez MS, Luza R, Alvarez P, Paredes F, Acevedo J, Farfán MJ, Solari S, Soto-Rifo R, Valiente-Echeverría F. Performance of SARS-CoV-2 rapid antigen test compared with real-time RT-PCR in asymptomatic individuals.
Int J Infect Dis. 2021 Jun;107:201-204. doi: 10.1016/j.ijid.2021.04.087.
6. Balcells ME, Rojas L, Le Corre N, Martínez-Valdebenito C, Ceballos ME, Ferrés M, Chang M, Vizcaya C, Mondaca S, Huete Á, Castro R, Sarmiento M, Villarroel L, Pizarro A, Ross P, Santander J, Lara B, Ferrada M, Vargas-Salas S, Beltrán-Pavez C, Soto-Rifo R, Valiente-Echeverría F, Caglevic C, Mahave M, Selman C, Gazitúa R, Briones JL, Villarroel-Espindola F, Balmaceda C, Espinoza MA, Pereira J, Nervi B. Early versus deferred anti-SARS-CoV-2 convalescent plasma in patients admitted for COVID-19: A randomized phase II clinical trial.
PLoS Med. 2021 Mar 3;18(3):e1003415. doi: 10.1371/journal.pmed.1003415
7. González-Stegmaier R, Cereceda K, Briones JL, Beltran-Pávez C, Oyarzún-Arrau A, Riquelme-Barrios S, Selman C, Yarad F, Mahave M, Caglevic C, Morales R, Aguirre A, Valiente-Echeverría F, Soto-Rifo R, Marsiglia H, Gazitua R, Villarroel-Espindola F. Seroconversion and Abundance of IgG Antibodies against S1-RBD of SARS-CoV-2 and Neutralizing Activity in the Chilean Population.
J Immunol Res. 2021 Feb 19;2021:6680337. doi: 10.1155/2021/6680337
8. Gaete-Argel A, Velásquez F, Márquez CL, Rojas-Araya B, Bueno-Nieto C, Marín-Rojas J, Cuevas-Zúñiga M, Soto-Rifo R, Valiente-Echeverría F. Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage.
Front Cell Dev Biol. 2021 Feb 11;9:622057. doi: 10.3389/fcell.2021.622057.
9. Beltrán-Pavez C, Riquelme-Barrios S, Oyarzún-Arrau A, Gaete-Argel A, González-Stegmaier R, Cereceda-Solis K, Aguirre A, Travisany D, Palma-Vejares R, Barriga GP, Gaggero A, Martínez-Valdebenito C, Corre NL, Ferrés M, Balcells ME, Fernandez J, Ramírez E, Villarroel F, Valiente-Echeverría F, Soto-Rifo R. Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile. Sci Adv. 2021 Feb 12;7(7):eabe6855. doi: 10.1126/sciadv.abe6855.
10. Velásquez F, Marín-Rojas J, Soto-Rifo R, Torres A, Del Canto F, Valiente-Echeverría F. Escherichia coli HS and Enterotoxigenic Escherichia coli Hinder Stress Granule Assembly. Microorganisms. 2020 Dec 23;9(1):17. doi: 10.3390/microorganisms9010017.
11. García-de-Gracia F, Gaete-Argel A, Riquelme-Barrios S, Pereira-Montecinos C, Rojas-Araya B, Aguilera P, Oyarzún-Arrau A, Rojas-Fuentes C, Acevedo ML, Chnaiderman J, Valiente-Echeverría F, Toro-Ascuy D, Soto-Rifo R. CBP80/20-dependent translation initiation factor (CTIF) inhibits HIV-1 Gag synthesis by targeting the function of the viral protein Rev. RNA Biol. 2020 Oct 25:1-14. doi: 10.1080/15476286.2020.1832375
12. Beltrán-Pavez C, Alonso-Palomares LA, Valiente-Echeverría F, Gaggero A, Soto-Rifo R, Barriga GP. Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction. J. Virol Methods. 2020 Sep 9:113969. doi: 10.1016/j.jviromet.2020.113969
13. Zhu L, Liu X, Huang H, Avellán-Llaguno RD, Lazo MML, Gaggero A, Soto-Rifo R, Patiño L, Valencia-Avellan M, Diringer B, Huang Q, Zhu YG. Meteorological impact on the COVID-19 pandemic: A study across eight severely affected regions in South America. Sci Total Environ. 2020 Jul 11;744:140881. doi: 10.1016/j.scitotenv.2020.140881
14. Órdenes-Aenishanslins N, Anziani-Ostuni G, Monrás JP, Tello A, Bravo D, Toro-Ascuy D, Soto-Rifo R, Prasad PN, Pérez-Donoso JM. Bacterial Synthesis of Ternary CdSAg Quantum Dots through Cation Exchange: Tuning the Composition and Properties of Biological Nanoparticles for Bioimaging and Photovoltaic Applications. Microorganisms. 2020 Apr 27;8(5). pii: E631. doi: 10.3390/microorganisms8050631
15. Oyarzún-Arrau A, Alonso-Palomares L, Valiente-Echeverría F, Osorio F, Soto-Rifo R. (2020) Crosstalk between RNA Metabolism and Cellular Stress Responses during Zika Virus Replication. Pathogens. 2020 Feb 25;9(3). pii: E158. doi: 10.3390/pathogens9030158. Review.
16. Dubois-Camacho K, Diaz-Jimenez D, De la Fuente M, Quera R, Simian D, Martínez M, Landskron G, Olivares-Morales M, Cidlowski JA, Xu X, Gao G, Xie J, Chnaiderman J, Soto-Rifo R, González MJ, Calixto A, Hermoso MA. Inhibition of miR-378a-3p by Inflammation Enhances IL-33 Levels: A Novel Mechanism of Alarmin Modulation in Ulcerative Colitis. Front Immunol. 2019 Nov 20;10:2449. doi: 10.3389/fimmu.2019.02449. eCollection 2019.
17. Gaete-Argel A, Márquez CL, Barriga GP, Soto-Rifo R, Valiente-Echeverría F. Strategies for Success. Viral Infections and Membraneless Organelles. Front Cell Infect Microbiol. 2019 Oct 11;9:336. doi: 10.3389/fcimb.2019.00336. eCollection 2019. Review
18. Rojas-Celis V, Valiente-Echeverría F, Soto-Rifo R, Toro-Ascuy D. New Challenges of HIV-1 Infection: How HIV-1 Attacks and Resides in the Central Nervous System. Cells. 2019 Oct 13;8(10). pii: E1245. doi: 10.3390/cells8101245. Review
19. Fuentes-Villalobos, Francisco; Farkas P., Carlos; Sebastian Riquelme-Barrios; Armijo, ME; Soto-Rifo, Ricardo; Pincheira, Roxana; Castro, Ariel F. DISC1 promotes translation maintenance during sodium arsenite-induced oxidative stress. Biochim Biophys Acta-Gene Regulatory Mechanisms. 2019 Jun;1862(6):657-669. doi: 10.1016/j.bbagrm.2019.05.001
20. Levicán-Asenjo J, Soto-Rifo R, Aguayo F, Gaggero A, Leon O. Salmon cells SHK-1 internalize infectious pancreatic necrosis virus by macropinocytosis. J Fish Dis. 2019 May 2
21. Daniela Toro-Ascuy; Bárbara Rojas-Araya; Francisco García-de-Gracia; Cecilia Rojas-Fuentes; Camila Pereira-Montecinos; Aracelly Gaete-Argel; Fernando Valiente-Echeverría; Théophile Ohlmann, Ricardo Soto-Rifo. A Rev-CBP80-eIF4AI complex drives Gag synthesis from the HIV-1 unspliced mRNA. Nucleic Acids Res. 2018 Nov 30;46(21):11539-11552.
22. Acevedo ML, García-de Gracia F, Miranda-Cárdenas C, Soto-Rifo R, Aguayo F, León O. Differences in the internalization of self-inactivating VSVG-pseudotyped murine leukemia virus-based vectors in human and murine cells. J Virol Methods. 2018 May;255:14-22
23. Riquelme-Barrios S, Pereira-Montecinos C, Valiente-Echeverría F, Soto-Rifo R. Emerging Roles of N6-Methyladenosine on HIV-1 RNA Metabolism and Viral Replication. Front Microbiol. 2018 Mar 28;9:576
24. Levican J, Miranda-Cárdenas C, Soto-Rifo R, Aguayo F, Gaggero A, León O. Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis. Sci Rep. 2017 Jun 8;7(1):3068
25. Pereira-Montecinos C, Valiente-Echeverría F, Soto-Rifo R. Epitranscriptomic regulation of viral replication. Biochim Biophys Acta-Gene Regulatory Mechanisms. 2017 Apr;1860(4):460-471.
26. Mengardi C, Limousin T, Ricci EP, Soto-Rifo R, Decimo D, Ohlmann T. microRNAs stimulate translation initiation mediated by HCV-like IRESes. Nucleic Acids Res. 2017 May 5;45(8):4810-4824.
27. Toro-Ascuy D, Rojas-Araya B, Valiente-Echeverría F, Soto-Rifo R. Interactions between the HIV-1 Unspliced mRNA and Host mRNA Decay Machineries. Viruses. 2016. Nov 23;8(11). pii: E320. Review.
28. Natalia Poblete-Durán, Yara Prades-Pérez, Jorge Vera-Otarola, Ricardo Soto-Rifo and Fernando Valiente-Echeverría. Who Regulates Whom? An Overview of RNA Granules and Viral Infections. Viruses. 2016. Jun 8(7), pii: E180
29. Alvaro Fröhlich, Bárbara Rojas-Araya, Camila Pereira-Montecinos, Alessandra Dellarossa, Daniela Toro-Ascuy, Yara Prades-Pérez, Francisco García-de-Gracia, Andrea Garcés-Alday, Paulina S. Rubilar, Fernando Valiente-Echeverría, Théophile Ohlmann and Ricardo Soto-Rifo. DEAD-box RNA helicase DDX3 connects CRM1-dependent nuclear export and translation of the HIV-1 unspliced mRNA through its N-terminal domain. Biochimica et Biophysica Acta-Gene Regulatory Mechanisms. 2016. Mar 21;1859(5):719-730.
30. Lara Ajamian, Karen Abel, Shringar Rao, Kishanda Vyboh, Francisco García-de-Gracia, Ricardo Soto-Rifo, Andreas Kulozik, Niels Gehring, Andrew Mouland. HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA. Biomolecules. 2015. 5:2808-2839.
31. Bárbara Rojas-Araya, Théophile Ohlmann* and Ricardo Soto-Rifo*. Translational control of the HIV-1 unspliced genomic mRNA. Viruses. 2015. Aug 4;7(8):4326-51. *Corresponding authors
32. Fernando Valiente-Echeverría*, Marcela Hermoso, Ricardo Soto-Rifo*. RNA helicase DDX3: at the crossroad of viral replication and antiviral immunity. Reviews in Medical Virology. 2015. Sep; 25(5): 286-99. *Corresponding authors
33. Peña N, Carrillo D, Muñoz JP, Chnaiderman J, Urzúa U, León O, Tornesello ML, Corvalán AH, Soto-Rifo R, Aguayo F. Tobacco Smoke Activates Human Papillomavirus 16 p97 Promoter and Cooperates with High-Risk E6/E7 for Oxidative DNA Damage in Lung Cells. Plos One. 2015. Apr; 1;10(4):e0123029
34. Ricardo Soto-Rifo*, Fernando Valiente-Echeverria, Paulina S. Rubilar, Francisco García-de-Gracia, Emiliano P. Ricci, Taran Limousin, Didier Décimo, Théophile Ohlmann*. HIV-2 genomic RNA accumulates in stress granules in the absence of active translation. Nucleic Acids Research. 2014. Nov;42(20):12861-75. *Corresponding authors
35. Ricardo Soto-Rifo*, Paulina S. Rubilar, Théophile Ohlmann*. The DEAD-box helicase DDX3 substitutes for the cap-binding protein eIF4E to promote compartmentalized translation initiation of the HIV-1 genomic RNA. Nucleic Acids Research. 2013. Jul;41(12):6286-99. *Corresponding authors
36. Ricardo Soto-Rifo* and Théophile Ohlmann. The roles of DDX3 in mRNA metabolism. Wiley Interdiscip Rev RNA. 2013 Jul-Aug;4(4):369-85. *Corresponding authors
37. Sylvain de Breyne, Ricardo Soto-Rifo, Marcelo Lopez-Lastra, Théophile Ohlmann. Translation initiation is driven by different mechanisms on the HIV-1 and HIV-2 genomic RNAs. Virus Research. 2013 Feb;171(2):366-81.
38. Emiliano P Ricci*, Taran Limousin*, Ricardo Soto-Rifo*, Didier Decimo, Theophile Ohlmann. miRNA repression of translation in vitro takes place during 43S ribosomal scanning. Nucleic Acids Research. 2013. Jan 7;41(1):586-98. *Joint first authors, equal contribution.
39. Ricardo Soto-Rifo, Taran Limousin, Paulina S. Rubilar, Sylvain de Breyne, Didier Décimo, Théophile Ohlmann. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J. 2012. Aug 7;31(18):3745-56.
40. Jorge Vera-Otarola, Loretto Solis, Ricardo Soto-Rifo, Emiliano Ricci, Karla Pino, Nicole Tischler, Théophile Ohlmann, Jean-Luc Darlix, Marcelo Lopez-Lastra. The Andes Hantavirus NSs protein is expressed from the viral Small mRNA by a leaky scanning mechanism. J Virol. 2012. Feb;86(4):2176-87.
41. Ricardo Soto-Rifo, Taran Limousin, Paulina S. Rubilar, Emiliano P. Ricci, Didier Décimo, Olivier Moncorgé, Mary-Anne Trabaud, Patrice André, Andrea Cimarelli, Théophile Ohlmann. Different effects of the TAR structure on HIV-1 and HIV-2 genomic RNA translation. Nucleic Acids Res. 2012. Mar;40(6):2653-67.
42. Sandrine Alais, Ricardo Soto-Rifo, Vincent Balter, Henri Gruffat, Evelyne Manet, Laurent Schaeffer, Jean Luc Darlix, Andrea Cimarelli , Graça Raposo, Théophile Ohlmann, Pascal Leblanc. Functional mechanisms of the cellular prion protein (PrPC) associated anti-HIV-1 properties. Cell. Mol. Life Sci. 2011. Apr;69(8):1331-52.
43. Ricci EP, Limousin T, Soto-Rifo R, Alisson R, Poÿry T, Decimo D, Jackson RJ, Ohlmann T. Activation of a microRNA response in trans reveals a new role for the poly(A) tail in translational repression. Nucleic Acids Res. 2011. Jul;39(12):5215-31.